![]() There are a wide selection of Mini-PROTEAN Combs, available in 0.75 mm, 1.0 mm, and 1. It also preserves your precious sample too.ĭiscover how Bio-Rad StarBright™ Blue 700 Secondary Antibodies and ChemiDoc™ MP Imaging System can aid your fluorescent multiplexing. Choose these Mini-PROTEAN 15-well combs for use with spacer plates with integrated 1.5 mm spacers for preparing gels with the Mini-PROTEAN Tetra Handcast Systems and Mini-PROTEAN 3 Multi-Casting Chamber.Packaging Options. Thereby enabling direct protein abundance comparisons and normalization against a control, for instance a housekeeping gene, which is more accurate, easier and time saving as you will no longer have to strip and re-probe. Multiplexing fluorescent western blotting enables multiple proteins to be detected and quantified in a single sample with highly specific and sensitive fluorescent antibodies. Western blotting products include protein transfer systems, blotting membranes, filter paper, premixed blotting buffers and reagents, and detection kits. Preset configurations can be ordered with the following catalog numbers: 165-8000 (10-well combs, 0.75 mm spacer plates) or 165-8001 (10-well combs, 1.0 mm spacer plates). Also available is our Western Blot Doctor™, a self-help guide to identify and troubleshoot western blotting problems. Our introduction to western blotting guide provides in depth, step by step information on this ubiquitous technique. Using the Trans-Blot SD Semi-Dry Transfer Cell. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells or tissue. Remove comb: Position both thumbs on the ridges on the comb. Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology. Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins Ideal for use with Trans-Blot Turbo preassembled transfer packs. Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues. Holds 1 midi-gel or 2 mini-gel blotting sandwiches. Western Blot Detection of Phosphorylation Events Use the Trans-Blot Turbo cassette for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system.
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